Biotinylated Srctide Multi-Kinase Substrate

Biotin-GEEPLYWSFPAKKK-NH2 is an N-terminally biotinylated derivative of Srctide, a broad protein-tyrosine-kinase substrate. The central tyrosine is phosphorylated by numerous Src-family, Tec-family and receptor tyrosine kinases, while the biotin group provides an affinity handle for streptavidin capture, immobilization and solid-phase assay formats.

• protein tyrosine kinase activity assays
• streptavidin-based phosphorylation assays
• Src/Tec/receptor kinase profiling
• kinase inhibitor screening

Srctide is a broad multi-kinase substrate and does not identify a single kinase in complex samples. Biotinylation can also alter surface accessibility and kinetics; use controls appropriate to the immobilized or captured assay format.

1. Characterization of Phosphorylation Status and Kinase Activity of Src Family Kinases Expressed in Cell-Based and Cell-Free Protein Expression Systems
2. Bruton's tyrosine kinase inhibitor BMS-986142 in experimental models of rheumatoid arthritis enhances efficacy of agents representing clinical standard-of-care