Biotinylated AKTide-2T Akt/PKB Substrate

Biotin-ARKRERTYSFGHHA is a biotinylated derivative of AKTide-2T, an optimized peptide substrate for protein kinase B/Akt. Library screening identified the ARKRERTYSFGHHA motif as a favorable Akt/PKB phosphorylation sequence, with the serine serving as the principal phosphoacceptor. The N-terminal biotin provides an affinity handle for streptavidin capture and solid-phase kinase-assay formats.

• Akt/PKB kinase activity assays
• streptavidin-based kinase assays
• Akt substrate-recognition studies
• kinase inhibitor screening

Biotinylation creates a distinct reagent from unconjugated AKTide-2T. Binding to streptavidin surfaces and tag accessibility can influence apparent kinetics, so assay conditions should be validated for the conjugated peptide.

1. Peptide and Protein Library Screening Defines Optimal Substrate Motifs for AKT/PKB