|Beta-Amyloid (1-42), human|
| ||1 mg, 95.21% Purity |
|Beta amyloid 1-42 is known as a biomarker of Alzheimer's disease. Amyloid is detectable in cerebrospinal fluid (CSF). It is a 42-amino acid fragment of amyloid precursor protein. The peptide is well suited for use as a standard in the quantitation of Alzheimer's. Amyloid (1-42), a major component of amyloid plaques, accumulates in neurons of Alzheimer’s disease brains. Biochemical analysis of the amyloid peptides isolated from Alzheimer's disease brain indicates that Aß (1-42) is the principal species associated with senile plaque amyloids, while Aß (1-40) is more abundant in cerebrovascular amyloid deposit.|
How to form the fibrillary structure using beta-amyloid peptides?
Amyloid (1-42) was dissolved to 1 mM in 100% hexafluoroisopropanol, hexafluoroisopropanol was removed under vacuum, and the peptide was stored at -20 C. For the aggregation protocols, the peptide was first resuspended in dry Me2SO (DMSO) to 5 mM. For oligomeric conditions, F-12 (without phenol red) culture media was added to bring the peptide to a final concentration of 100 uM, and the peptide was incubated at 4 C for 24 h. For fibrillar conditions, 10 mM HCl was added to bring the peptide to a final concentration of 100 uM, and the peptide was incubated for 24 h at 37 C. ADDLS, amyloid derived diffusible ligands.
R. Oyola, D. Du, I. Ramos, K. Torres, A. S. Delgado, E. R. Serrano, A. Meléndez and N. V. Falcón-Cruz, Mater. Adv., 2021, DOI:10.1039/D1MA00461A.
... AB40 was obtained from Life Tein Company (NJ, USA). Certified analysis of
the peptide established that the purity is higher than> 95% with formula weight of 4329.9 …