| Beta-Amyloid (1-42), human |
| 1 mg, 95.21% Purity |
| Beta amyloid 1-42 is known as a biomarker of Alzheimer's disease. Amyloid is detectable in cerebrospinal fluid (CSF). It is a 42-amino acid fragment of amyloid precursor protein. The peptide is well suited for use as a standard in the quantitation of Alzheimer's. Amyloid (1-42), a major component of amyloid plaques, accumulates in neurons of Alzheimer’s disease brains. Biochemical analysis of the amyloid peptides isolated from Alzheimer's disease brain indicates that Amyloid(1-42) is the principal species associated with senile plaque amyloids, while Aß (1-40) is more abundant in cerebrovascular amyloid deposit. |
| LT2460 |
| 4514.14 |
| C203H311N55O60S1 |
| Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala, DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA, |
How to form the fibrillary structure using beta-amyloid peptides?
Amyloid (1-42) was dissolved to 1 mM in 100% hexafluoroisopropanol, hexafluoroisopropanol was removed under vacuum, and the peptide was stored at -20 C. For the aggregation protocols, the peptide was first resuspended in dry Me2SO (DMSO) to 5 mM. For oligomeric conditions, F-12 (without phenol red) culture media was added to bring the peptide to a final concentration of 100 uM, and the peptide was incubated at 4 C for 24 h. For fibrillar conditions, 10 mM HCl was added to bring the peptide to a final concentration of 100 uM, and the peptide was incubated for 24 h at 37 C. ADDLS, amyloid derived diffusible ligands.
Reference:
R. Oyola, D. Du, I. Ramos, K. Torres, A. S. Delgado, E. R. Serrano, A. Meléndez and N. V. Falcón-Cruz, Mater. Adv., 2021, DOI:10.1039/D1MA00461A.
... AB40 was obtained from Life Tein Company (NJ, USA). Certified analysis of the peptide established that the purity is higher than> 95% with formula weight of 4329.9 …
