Anti-Biotin Rabbit Monoclonal Antibody (PE Conjugate)
High-sensitivity detection of biotinylated LPETG substrates in Sortase A-mediated labeling systems
Product Overview
The Anti-Biotin Rabbit Monoclonal Antibody (PE conjugated) is designed for highly sensitive detection of
biotin-labeled proteins, peptides, and cell-surface interactions. This antibody is particularly optimized
for applications involving Sortase A (SrtA)-mediated labeling using LPXTG motif-containing substrates
such as Biotin-Ahx-LPETGS-NH2.
When used together with recombinant Sortase A, this antibody enables robust detection of
proximity-dependent labeling systems including LIPSTIC assays, immune synapse mapping,
and dynamic cell–cell interaction studies.
Key Features
- Rabbit monoclonal antibody – high specificity and low background
- PE conjugate – ideal for flow cytometry and fluorescence detection
- Optimized for Sortase A labeling workflows
- Superior signal compared to streptavidin-based detection in cellular assays
- Validated for detecting LPETG-mediated biotin transfer
Specifications
| Cat #: | LT8311 |
| Host Species: | Rabbit |
| Clonality: | Monoclonal |
| Conjugation: | Phycoerythrin (PE) |
| Reactivity: | Biotin |
| Applications: | Flow Cytometry, Cell Labeling, Protein Detection |
| Unit Size: | 100 μg per vial |
| Storage: | Store at 2–8°C, protected from light |
Applications
- LIPSTIC (Labeling Immune Partnerships by SorTagging Intercellular Contacts)
- Sortase A-mediated protein labeling
- Cell–cell interaction mapping
- Flow cytometry detection of biotinylated targets
- Immunology and CAR-T studies
Recommended Workflow
Complete Sortase A labeling system:
- Label cells or proteins using Biotin-Ahx-LPETGS-NH2
- Perform enzymatic transfer using Recombinant Sortase A
- Detect biotin labeling using this Anti-Biotin-PE antibody
Why Use This Instead of Streptavidin?
- Reduced steric hindrance in cellular systems
- Better performance in live-cell assays
- Avoids endogenous biotin interference
- Compatible with multiplex fluorescence panels
Related Products
References
Pasqual et al., Nature 2018 – Monitoring T cell–dendritic cell interactions by enzymatic labeling
Nakandakari-Higa et al., Nature 2024 – Universal recording of immune cell interactions
Science Immunology 2024 – Tumor-specific CD4+ T cell response tracking
