Sortase Labeling Starter Kit

Sortase Labeling Starter Kit

All-in-one solution for Sortase A-mediated biotin labeling and flow cytometry detection

Build a complete LPETG / Sortase A workflow with substrate, enzyme, and fluorescent detection—optimized for LIPSTIC assays, cell–cell interaction studies, and protein labeling experiments.

$999.00 $1150.00 Save 15%

Kit Components

Cat #: LT8312
Biotin Substrate Biotin-Ahx-LPETGS-NH2
100 mg, >95% purity
Sortase A recognition substrate (LPETG motif)
Enzyme Recombinant Sortase A
100 μg
Catalyzes LPXTG-mediated ligation
Detection Anti-Biotin Rabbit mAb (PE)
100 μg
Flow cytometry-ready fluorescent detection

How It Works

  1. Labeling: Biotin-LPETGS tags donor cells or proteins
  2. Transfer: Sortase A transfers biotin to target proteins
  3. Detection: Anti-biotin-PE antibody enables fluorescence readout

This system enables direct detection of cell-cell interactions and protein modification events in real time.

Why This Kit?

✔ Complete Workflow
No need to source from multiple vendors
✔ Optimized Components
All reagents validated together
✔ Higher Sensitivity
PE detection improves signal vs. traditional methods
✔ Ideal for LIPSTIC
Designed for immune interaction mapping

Applications

  • LIPSTIC (cell-cell interaction tracking)
  • Flow cytometry detection of biotin labeling
  • CAR-T and immunology studies
  • Sortase A-mediated protein conjugation
  • Cell surface labeling and tracking

Expand Your Workflow

Why Not Just Use Streptavidin?

Feature Anti-Biotin mAb-PE Streptavidin
Signal intensity High (PE fluorophore) Moderate
Background Low Higher
Multiplex compatibility Excellent Limited
Live-cell use Better Limited

Start Your Sortase Labeling Experiment Today

This kit provides everything you need to move from substrate labeling to detection in a single purchase.

Bundle Price: $999.00

References

Nature (2018), Nature (2024), Science Immunology (2024) — Sortase-mediated labeling and immune interaction studies.

  • 20 Units in Stock
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