1. Design a biotin-labeled peptide library: overlapping or Alanine scan
2. The peptide library is coated on the avidin coated 96 well plates.
3. Peptide library is used to evaluate the binding of each peptide to different cell lines using CyQUANT Cell Proliferation Assays.
4. Flow cytometry: Determine the cellular uptake of selected FITC-labeled peptides.
5. Competitive assay: The cells are incubated with FITC-peptide with or without excess unlabeled peptide (100-fold)
6. Finetune the peptide sequence: structure analysis, cyclization, and D amino acid modification.
7. Find the promising receptor specific cancer cell binding peptide that can be conjugated directly to a chemotherapeutic drug or to nanoparticles for targeted drug delivery to enhance the efficacy of chemotherapy for cancer treatment.

Reference: https://www.nature.com/articles/s41598-019-38574-y