Catalog Number | LT8833 |
Product Name | Urokinase/Plasminogen Activator Fluorogenic Substrate, Z-Gly-Gly-Arg-AFC |
Product Quantity | 10 mg |
Sequence | Z-Gly-Gly-Arg-AFC |
Molecular Weight | 633.6 |
Formula | C28H30F3N7O7 |
Purity | ≥95% |
Scientific Background | Z-Gly-Gly-Arg-AFC is a fluorogenic arginine-containing substrate used for urokinase/plasminogen-activator and acrosin-related serine-protease measurements. Enzymatic cleavage releases AFC and permits sensitive fluorescence-based activity measurements. The Z-Gly-Gly-Arg recognition sequence has a long history in plasminogen-activator assays, and AFC derivatives provide a convenient fluorescent readout. |
Research Applications | - urokinase activity assays
- plasminogen-activator studies
- acrosin-related protease research
- serine-protease inhibitor screening
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Experimental Notes | Published AFC-substrate work identifies Z-Gly-Gly-Arg-AFC as a urokinase substrate. Related arginine-cleaving proteases can also hydrolyze short peptide substrates, so enzyme controls are recommended. |
Selected References | - The use of substrates with 7-amino-3-trifluoromethylcoumarine (AFC) leaving group in the localization of protease activities in situ
- Studies on tissue culture plasminogen activator II
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