Catalog Number | LT8949 |
Product Name | SARS-CoV-2 Spike S2 FRET Substrate |
Product Quantity | 0.1 mg |
Sequence | QXL®520-SKPSKRSFIED-K(5-FAM)-NH2 |
Molecular Weight | 2270.4 |
Purity | ≥95% |
Scientific Background | QXL520-SKPSKRSFIED-K(5-FAM)-NH2 is a quenched fluorescent peptide modeling the SARS-CoV-2 spike S2' protease-processing region. The KR-SF sequence spans a basic cleavage motif implicated in activation of the fusion machinery. The QXL520/5-FAM reporter pair provides a fluorescence readout when proteolysis separates quencher and fluorophore. |
Research Applications | - SARS-CoV-2 S2' cleavage research
- viral-entry protease studies
- fluorogenic protease assays
- protease inhibitor screening
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Experimental Notes | The peptide reproduces only the local S2' sequence and not the tertiary structure or glycosylation of native spike. Cleavage patterns may therefore vary among furin, TMPRSS2 and other proteases and should be verified experimentally. |
Selected References | - The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade
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