Catalog Number | LT8950 |
Product Name | SARS-CoV-2 Spike Sensitive FRET Substrate |
Product Quantity | 0.1 mg |
Sequence | FAM-QTNSPRRARSVASQSK-QXL520™-NH2 |
Molecular Weight | 2622.8 |
Purity | ≥95% |
Scientific Background | FAM-QTNSPRRARSVASQSK-QXL520-NH2 is an extended internally quenched substrate centered on the SARS-CoV-2 spike S1/S2 polybasic cleavage region. The sequence includes the QTNSPRRARSVASQS context surrounding the RRAR motif and places FAM and QXL520 at opposite ends of the reporter construct. Cleavage produces a fluorescence increase suitable for protease-activity and inhibitor-screening studies. |
Research Applications | - spike S1/S2 protease studies
- furin-sensitive substrate assays
- SARS-CoV-2 inhibitor screening
- fluorescent protease method development
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Experimental Notes | This extended construct differs in sequence length and reporter orientation from the shorter S1/S2 substrate. Quantitative comparisons should therefore use matched substrate chemistry rather than assuming identical kinetics. |
Selected References | - The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade
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