Catalog Number | LT8948 |
Product Name | SARS-CoV-2 Spike S1/S2 FRET Substrate |
Product Quantity | 0.1 mg |
Sequence | QXL®520-TNSPRRARSVAS-K(5-FAM)-NH2 |
Molecular Weight | 2278.4 |
Purity | ≥95% |
Scientific Background | QXL520-TNSPRRARSVAS-K(5-FAM)-NH2 is an internally quenched peptide representing the SARS-CoV-2 spike S1/S2 junction. The sequence contains the polybasic RRAR cleavage motif introduced at the S1/S2 boundary. A QXL520 quencher and 5-FAM reporter enable fluorescence increase after proteolytic processing, providing a defined reagent for studies of furin-like cleavage and spike-processing proteases. |
Research Applications | - SARS-CoV-2 spike cleavage studies
- furin-like protease assays
- viral-entry protease research
- protease inhibitor screening
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Experimental Notes | This peptide models a short cleavage-site segment rather than the conformational environment of trimeric spike protein. Protease specificity and cleavage efficiency should therefore be established for the enzyme and buffer system used. |
Selected References | - The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade
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