Catalog Number | LT8808 |
Product Name | Renin FRET Substrate II, R-E(EDANS)-IHPFHLVIHT-K(DABCYL)-R |
Product Quantity | 1 mg |
Sequence | R-E(EDANS)-IHPFHLVIHT-K(DABCYL)-R |
Purity | ≥95% |
Scientific Background | R-E(EDANS)-IHPFHLVIHT-K(DABCYL)-R is an internally quenched renin substrate containing the human angiotensinogen-derived IHPFHLVIHT recognition sequence. Renin cleavage within this region separates EDANS from DABCYL and increases fluorescence, enabling continuous measurements of enzyme activity. The construct provides a chemically distinct reporter configuration from the terminally labeled DABCYL-gamma-Abu-IHPFHLVIHT-EDANS substrate. |
Research Applications | - renin activity assays
- renin inhibitor screening
- renin-angiotensin system research
- FRET protease method development
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Experimental Notes | This construct is chemically distinct from other IHPFHLVIHT renin substrates because EDANS and DABCYL are attached through side-chain handles. Do not merge their kinetic specifications without experimental validation. |
Selected References | - A continuous fluorescence assay of renin activity
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