| LT1691 |
| 1 MG |
| Recombinant Human IL‐6 (Interleukin ‐6) |
| Cartilage-inducing factor, Differentiation inhibiting factor |
| Human |
| Recombinant Human IL6 is a 20 to 28 kDa globular protein consisting of 212 amino acid residues. IL‐6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. It stimulates B cell differentiation and antibody production, synergizes with IL‐3 in megakaryocyte development and platelet production, induces expression of hepatic acute‐phase proteins, and regulates bone metabolism. IL‐6 signals through the IL‐6 receptor system that consists of two chains, IL‐6Rα and gp130. Murine IL‐ 6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant Human IL‐6 is a 20 to 28 kDa protein containing 212 amino acid residues. |
| APVPPGEDSK DVAAPHRQPL TSSERIDKQI RYILDGISAL RKETCNKSNM CESSKEALAE NNLNLPKMAE KDGCFQSGFN EETCLVKIIT GLLEFEVYLE YLQNRFESSE EQARAVQMST KVLIQFLQKK AKNLDAITTP DPTTNASLLT KLQAQNQWLQ DMTTHLILRS FKEFLQSSLR ALRQM |
| 21-23 kDA, glycosylated |
| Chinese Hamster Ovary cells |
| >95%, Determined by SDS‐PAGE under reducing conditions and visualized by silver stain. |
| Endotoxin Level < 1.0 EU per 1 μg of the protein by the LAL method |
| Sterile filtered 0.22 um filter in 20 mM TRIS*HCl buffer pH 7.2. Activated in vitro at pH below 3.5 and over 12.5. |
| ED50 < 0.6 ng/ml, determined by the dose dependent stimulation of the proliferation of human TF‐1 cells (human erythroleukemic indicator cell line) |
|  |
| ‐20°C to ‐80°C |
| For research purposes only! Not for human or animal therapeutic or diagnostic use. |
