Catalog Number | LT8933 |
Product Name | Plasminogen Activator/Acrosin Fluorogenic Substrate, Z-GGR-AFC |
Product Quantity | 10 mg |
Sequence | Z-GGR-AFC |
Molecular Weight | 633.6 |
Formula | C28H30F3N7O7 |
Purity | ≥95% |
Scientific Background | Z-Gly-Gly-Arg-AFC is a fluorogenic arginine-terminal peptide substrate used for plasminogen activator and acrosin-related serine-protease assays. Enzymatic cleavage releases AFC, enabling sensitive fluorescence detection. The Gly-Gly-Arg motif is a compact recognition sequence historically used in assays of urokinase-type plasminogen activator and related trypsin-like proteases. |
Research Applications | - plasminogen activator assays
- acrosin research
- fibrinolytic protease studies
- serine-protease inhibitor screening
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Experimental Notes | Arginine-directed serine proteases can share substrate preferences, so specificity depends on the biological preparation. Use enzyme-selective controls when applying this substrate to complex samples. |
Selected References | - Studies on tissue culture plasminogen activator II. The detection and assay of urokinase and plasminogen activator from LLC-PK1 cultures by a synthetic substrate
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