Catalog Number | LT8807 |
Product Name | Plasmepsin II Hemoglobin FRET Substrate 2837b |
Product Quantity | 1 mg |
Sequence | EDANS-CO-CH2-CH2-CO-ALERMFLSFP-Dap(DABCYL)OH |
Molecular Weight | 1896.0 |
Purity | ≥95% |
Scientific Background | This EDANS/DABCYL-labeled peptide is a fluorogenic substrate for Plasmodium plasmepsins based on the major hemoglobin alpha-chain cleavage region. Plasmepsins participate in hemoglobin degradation within malaria parasites, and the ALERMFLSFP sequence spans the region surrounding the Phe-Leu cleavage site. Reporter separation following proteolysis enables continuous fluorescence-based enzyme measurements. |
Research Applications | - plasmepsin II activity assays
- malaria aspartic-protease research
- hemoglobin-cleavage studies
- plasmepsin inhibitor screening
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Experimental Notes | Plasmepsins are acidic aspartic proteases, so assay pH has a strong effect on activity. Protect the EDANS/DABCYL substrate from light and compare inhibitor data under matched buffer conditions. |
Selected References | - Plasmepsin II hemoglobin-degradation studies
- Expression and enzymatic characterization of soluble recombinant plasmepsin I
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