Catalog Number | LT8980 |
Product Name | OVA (257-264), 5-FAM-Labeled |
Product Quantity | 1 mg |
Sequence | 5-FAM-SIINFEKL-NH2 |
Molecular Weight | 1320.5 |
Formula | C66H85N11O18 |
Purity | ≥95% |
Scientific Background | 5-FAM-SIINFEKL-NH2 is a fluorescent derivative of the canonical ovalbumin OVA(257-264) epitope SIINFEKL. SIINFEKL is presented by murine H-2Kb MHC class I and is one of the most widely used model antigens for studying CD8 T-cell recognition, antigen presentation and OT-I T-cell biology. |
Research Applications | - H-2Kb antigen-presentation studies
- OT-I/CD8 T-cell assays
- fluorescent peptide uptake
- MHC class I immunology
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Experimental Notes | This product is C-terminally amidated and N-terminally FAM labeled, so it is chemically distinct from free-terminal SIINFEKL. Labeling and amidation can affect MHC loading and uptake and should be controlled experimentally. |
Selected References | - A Role for CD8 in the Developmental Tuning of Antigen Recognition and CD3 Conformational Change
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