Ni Sepharose Fast Flow

Catalog Number:
Packing Details:
1 ml prepacked column
6% highly cross-linked spherical agarose
Ni Sepharose Fast Flow
Ligand Density:
~40 mg histidine-tagged protein
Particle Size:
45~165 μm
Maximum Flow Rate:
300 cm/h
Metal Ion Capacity:
~15 µmol Ni2₊/ml medium
pH Limits:
pH 2-14
4 °C to 8 °C in 20% ethanol

Ni Sepharose Fast Flow is used for capture and intermediate purification. Ni Sepharose Fast Flow has a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2₊ ions for purification of histidine tagged proteins. Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea

  1. Use the prepacked column for easier process.
  2. The target molecule should be stable under the selected elution conditions.
  3. Avoid air bubbles in the column.
  4. It is recommended to regenerate the resin by 4 column volumes of 0.1 M glycine-HCl (pH 2.7) after 5 separation cycles.
  5. After 10 separation cycles, wash the resin with 5 column volumes of 20% ethanol and 5~10 column volumes of 70% ethanol sequentially to remove hydrophobic substances.
  6. For longer periods of storage, keep at 4~8ºC in 20% ethanol.
For research purposes only. Not for human or animal therapeutic or diagnostic use.
  • 5 Units in Stock

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