Catalog Number | LT8837 |
Product Name | MMP-2/MMP-9 Colorimetric Thiol Peptide Substrate |
Product Quantity | 1 mg |
Sequence | Ac-PLG-SCH[CH2CH(CH3)2]-CO-LG-OC2H5 |
CAS Number | 98992-65-5 |
Molecular Weight | 655.9 |
Formula | C31H53N5O8S |
Purity | ≥95% |
Scientific Background | Ac-PLG-SCH[CH2CH(CH3)2]-CO-LG-OC2H5 is a thiol-containing synthetic substrate developed for continuous spectrophotometric assays of vertebrate collagenases and gelatinases. Enzymatic cleavage exposes a thiol-containing product that can be detected with thiol-reactive chromogenic reagents such as 4,4'-dithiodipyridine or Ellman's reagent. The substrate has been used in MMP-2/MMP-9-related assay formats and in foundational collagenase substrate studies. |
Research Applications | - MMP-2/MMP-9 activity assays
- collagenase/gelatinase kinetics
- colorimetric metalloproteinase assays
- MMP inhibitor screening
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Experimental Notes | This is a chemically modified thiol substrate rather than a conventional unmodified peptide. Color development requires an appropriate thiol-reactive indicator reagent, and kinetic parameters depend on the enzyme and assay conditions. |
Selected References | - Spectrophotometric assay for vertebrate collagenase
- Synthetic substrates of vertebrate collagenase
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