MMP-12 FRET Substrate, Mca-PLGLEEA-Dap(Dnp)-NH2

Mca-PLGLEEA-Dap(Dnp)-NH2 is an internally quenched fluorogenic substrate developed for matrix metalloproteinase-12 (macrophage elastase). Proteolytic cleavage separates the Mca fluorophore from the Dnp quencher and increases fluorescence, enabling continuous MMP-12 activity measurements. The substrate was developed in studies aimed at improving selectivity for MMP-12 over related metalloproteinases.

• MMP-12 activity assays
• fluorogenic protease measurements
• MMP inhibitor screening
• metalloproteinase specificity studies

Protect the peptide from light and validate substrate concentration within the intended enzyme system. Apparent selectivity can vary with enzyme concentration and assay buffer.

1. Development of Selective Inhibitors and Substrate of Matrix Metalloproteinase-12