Kemptide, 5-FAM Labeled

5-FAM-LRRASLG is a fluorescent derivative of Kemptide, the classic heptapeptide substrate for cAMP-dependent protein kinase (PKA). The serine residue is the phosphate acceptor, while the N-terminal fluorescein reporter enables direct fluorescence detection and separation-based kinase assay formats. LifeTein lists excitation and emission maxima near 494 and 521 nm for this labeled reagent.

• fluorescent PKA activity assays
• cAMP-dependent kinase studies
• mobility-shift and separation assays
• PKA inhibitor screening

The FAM label changes chromatographic behavior and may alter apparent kinetics relative to unlabeled Kemptide. Protect from light and use matched fluorescent-substrate controls for quantitative comparisons.

1. Isolation and characterisation of cAMP-dependent protein kinase from Candida albicans