Insulin Receptor (1142-1153), pTyr1150

TRDIYETD-pY-YRK is a singly phosphorylated peptide from the insulin receptor activation-loop region, with phosphotyrosine corresponding to Tyr1150. This chemically defined peptide is useful for comparing site-specific insulin-receptor phosphorylation states, evaluating phosphotyrosine-binding or dephosphorylation assays, and developing phosphopeptide mass-spectrometry methods.

• insulin receptor phosphosite studies
• phosphotyrosine-binding assays
• protein tyrosine phosphatase research
• phosphoproteomics controls

This is a pre-phosphorylated Tyr1150 peptide. It should not be described as an unmodified kinase substrate, and its behavior should be distinguished from the triply phosphorylated 1146/1150/1151 activation-loop peptide.

1. Increasing the cAMP content of IM-9 cells alters the phosphorylation state and protein kinase activity of the insulin receptor