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| Human IL-10 R alpha Protein (LTP10379) |
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| LTP10379 |
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| 100ug |
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$473 In stock |
| The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne's disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD. |
| Recombinant Human IL-10 R alpha Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains His22-Asn235. |
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| IL-10R alpha |
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| Human |
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| Q13651 |
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| His22-Asn235 |
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| The protein has a predicted MW of 27 kDa. Due to glycosylation, the protein migrates to 40-48 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human IL-10, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 21.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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