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| Human IL-10 Protein (LTP10970) |
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| LTP10970 |
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| 100ug |
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$676 In stock |
| Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life. |
| Recombinant Human IL-10 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal. It contains Ser19-Asn178. |
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| IL-10 |
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| Human |
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| P22301 |
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| Ser19-Asn178 |
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| The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on the Tris-Bis PAGE result. |
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| The affinity constant of 0.13 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| N-His-Avi |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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