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| Human DDR1 Protein (LTP10281) |
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| LTP10281 |
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| 100ug |
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$406 In stock |
| Discoidin domain receptor1 (DDR1) is a collagen activated receptor tyrosine kinase and an attractive anti-fibrotic target. Its expression is mainly limited to epithelial cells located in several organs including skin, kidney, liver and lung. DDR1's biology is elusive, with unknown downstream activation pathways; however, it may act as a mediator of the stromal-epithelial interaction, potentially controlling the activation state of the resident quiescent fibroblasts. |
| Recombinant Human DDR1 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Asp21-Ala417. |
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| DDR1 |
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| Human |
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| Q08345-1 |
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| Asp21-Ala417 |
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| The protein has a predicted MW of 45.1 kDa. Due to glycosylation, the protein migrates to 63-67 kDa based on the Tris-Bis PAGE result. |
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| Immobilized Human DDR1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 12.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.806 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
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| C-His |
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| Expi293 |
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| > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
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| Less than 1EU per ug by the LAL method. |
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| Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization. |
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| Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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| Shipped at ambient temperature. |
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| Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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