Catalog Number | LT9084 |
| Product Name | Histone H4 (1-25) K12Ac/K16Ac-GSGSK(Biotin) |
| Product Quantity | 1 mg |
| Sequence | SGRGKGGKGLG-K(Ac)-GGA-K(Ac)-RHRKVLRDN-GSGSK(Biotin) |
| Molecular Weight | 3317 |
| Purity | ≥95% |
| Scientific Background | This histone H4(1-25) peptide carries acetylation at Lys12 and Lys16 and a C-terminal GSGSK(Biotin) affinity handle. H4K16 acetylation strongly influences chromatin interactions, while combined H4K12/K16 acetylation provides a defined diacetylated H4 state for studying bromodomain recognition, heterochromatin-associated interactions and histone acetylation crosstalk. |
| Research Applications |
- H4K12/K16 acetylation studies
- bromodomain and chromatin-reader binding
- heterochromatin protein interaction studies
- streptavidin pull-down assays
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| Experimental Notes | The two acetyl-lysine sites and the GSGSK(Biotin) extension are part of the exact reagent identity. This diacetylated construct should be compared with matched unmodified and single-acetyl H4(1-25) controls. |
| Selected References |
- Carmen et al., J Biol Chem. Histone H4 acetylation and SIR3 binding.
- Turner et al., FEBS Lett. Site-specific histone H4 acetylation.
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