Histone H3 (23-34) K27Me3-GGK(Biotin)

This H3K27me3 peptide combines the canonical repressive K27 trimethyl mark with a C-terminal GGK(Biotin) handle. The construct supports streptavidin immobilization and pull-down formats for Polycomb and other H3K27me3-binding proteins.

• H3K27me3 pull-down assays
• Polycomb reader studies
• streptavidin immobilization
• epigenetic binding screens

The added GGK(Biotin) linker changes the molecular weight and assay format relative to free H3K27me3. Use the corresponding unmodified or nonbiotinylated peptide as an appropriate control.

1. Polycomb-mediated methylation on Lys27 of histone H3 pre-marks genes for de novo methylation in cancer