Histone H3 (23-34) K27Me2

H3K27me2 is a broadly distributed Polycomb-associated histone modification and a major product state of PRC2 activity. This short site-specific peptide is useful for biochemical comparison with K27me1 and K27me3.

• H3K27me2 enzyme assays
• PRC2 methylation research
• reader-domain binding
• epigenetic inhibitor screening

Dimethylation at Lys27 creates a distinct molecular recognition state. Quantitative binding or enzyme results should be compared using otherwise matched H3(23-34) peptides.

1. Quantitative Mass Spectrometry of Histones H3.2 and H3.3 in Suz12-deficient Mouse Embryonic Stem Cells Reveals Distinct, Dynamic Post-translational Modifications at Lys-27 and Lys-36