Histone H3 (23-34) K27Me1

H3K27me1 is the monomethylated state of Lys27. The short H3(23-34) construct is useful for defining methylation-state-dependent recognition and for comparing the progressive mono-, di- and trimethylated K27 states.

• H3K27 methylation-state profiling
• PRC2-related studies
• histone reader binding
• epigenetic enzyme assays

The single methyl group at Lys27 is part of the exact chemical identity. Do not substitute H3K27me2 or H3K27me3 when evaluating methylation-state selectivity.

1. Quantitative Mass Spectrometry of Histones H3.2 and H3.3 in Suz12-deficient Mouse Embryonic Stem Cells Reveals Distinct, Dynamic Post-translational Modifications at Lys-27 and Lys-36