| Product Name | Histone H3 (1-21) K4Me1-GGK(Biotin) |
| Product Quantity | 0.25 mg |
| Sequence | ART-K(Me1)-QTARKSTGGKAPRKQLA-GGK(Biotin) |
| Molecular Weight | 2737.4 |
| Purity | ≥95% |
| Mechanism & Biological Significance | H3K4me1 is a monomethylated H3 state used to interrogate methylation-sensitive reader proteins and LSD1/KDM1A demethylase activity. Appending GGK(Biotin) enables capture and homogeneous detection while preserving the H3K4me1 substrate region. |
| Published Research Context | The exact the commercial supplier AS-64355 sequence has been used directly in LANCE-format LSD1 demethylase assays at 0.4 micromolar substrate concentration, with streptavidin-based detection of the biotinylated peptide. The same catalog peptide has also been used in modern histone-peptide pull-down studies to compare PHF23 binding across H3K4 methylation states. |
| Research Applications | - LSD1/KDM1A demethylase assays
- epigenetic inhibitor screening
- H3K4 methyl-state reader binding
- streptavidin pull-down and homogeneous detection
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| Experimental Considerations | This free-acid GGK(Biotin) construct is chemically distinct from GGK(Biotin)-NH2 or fluorescent H3K4me1 variants. Maintain the exact methylation state and terminal chemistry in comparative assays. |
| Selected Scientific References | - PubChem LSD1 histone-demethylase assay using the commercial supplier 64355
- PHF23 histone peptide pull-down study using AS-64355-025
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