| Product Name | Histone H3 (1-10) K4Me2 |
| Product Quantity | 1 mg |
| Sequence | ART-K(Me2)-QTARKS |
| Molecular Weight | 1174.4 |
| Purity | ≥95% |
| Mechanism & Biological Significance | Histone H3K4me2 is the dimethylated Lys4 state of the H3 N-terminal tail. The 10-residue peptide is a compact methyl-lysine ligand for reader-domain binding and methylation-state discrimination studies. |
| Published Research Context | Peer-reviewed structural and binding work on ING4 used synthetic H3 residues 1-10 (ARTKQTARKS) carrying all four Lys4 methylation states, directly including the H3(1-10)K4me2 free peptide. This provides exact fragment- and methylation-state support rather than inference from a longer H3 construct. |
| Research Applications | - PHD-finger methyl-lysine binding studies
- H3K4 methylation-state comparison
- isothermal titration calorimetry
- chromatin-reader structure-function studies
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| Experimental Considerations | The free N- and C-termini and the K4 dimethylation state are part of the reagent identity. Do not substitute H3K4me1, H3K4me3, longer H3 fragments, or biotinylated constructs without assay-specific validation. |
| Selected Scientific References | - Molecular Basis of Histone H3K4me3 Recognition by ING4
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