Catalog Number | LT8841 |
Product Name | HCV NS3/4A Protease FRET Substrate, 5-FAM/QXL 520 |
Product Quantity | 1 mg |
Sequence | Ac-DE-Dap(QXL520)-EE-Abu-ψ-[COO]AS-C(5-FAMsp)-NH2 |
Molecular Weight | 1913.8 |
Purity | ≥95% |
Scientific Background | Ac-DE-Dap(QXL520)-EE-Abu-ψ-[COO]AS-C(5-FAMsp)-NH2 is a highly sensitive internally quenched FRET substrate for hepatitis C virus NS3/4A protease. The sequence contains an engineered scissile ester linkage within an HCV protease recognition motif, together with a QXL 520 quencher and 5-FAM reporter. Cleavage by NS3/4A separates the reporter pair and produces a fluorescence increase suitable for continuous protease activity measurements. |
Research Applications | - HCV NS3/4A protease activity assays
- HCV protease inhibitor screening
- continuous FRET enzyme kinetics
- viral protease assay development
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Experimental Notes | The modified ester-containing linkage and reporter chemistry are integral to the high-sensitivity substrate design. Published and vendor assay conditions should not be transferred directly to unmodified HCV cleavage peptides. |
Selected References | - A continuous assay of hepatitis C virus protease based on resonance energy transfer depsipeptide substrates
- A high throughput assay of the hepatitis C virus nonstructural protein 3 serine proteinase
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