Catalog Number | LT8966 |
| Product Name | HCV NS3/4A Protease FRET Substrate, FAM/QXL520 |
| Product Quantity | 1 mg |
| Sequence | Ac-DE-Dap(QXL®520)-EE-Abu-ψ-[COO]AS-C(5-FAMsp)-NH2 |
| Molecular Weight | 1913.8 |
| Purity | ≥95% |
| Scientific Background | A highly modified depsipeptide FRET substrate for hepatitis C virus NS3/4A protease. QXL520 and 5-FAMsp flank an NS3/4A recognition sequence containing Abu and a ψ-[COO] linkage. Published resistance studies have used this exact substrate chemistry for Michaelis-Menten and inhibitor measurements. |
| Research Applications | - HCV NS3/4A activity assays
- antiviral inhibitor screening
- drug-resistance studies
- viral protease kinetics
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| Experimental Notes | The Abu residue, depsipeptide linkage, QXL520-Dap and 5-FAMsp-Cys are all part of the defined substrate and should not be simplified to an all-amide peptide. |
| Selected References | - Evaluating the Role of Macrocycles in the Susceptibility of Hepatitis C Virus NS3/4A Protease Inhibitors to Drug Resistance
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