Catalog Number | LT8842 |
Product Name | HCAM-2 Pertussis Toxin Substrate, 6-FAM-Gαi3C20 |
Product Quantity | 1 mg |
Sequence | 6-FAM-VFDAVTDVIIKNNLKECGLY |
Molecular Weight | 2613.1 |
Purity | ≥95% |
Scientific Background | 6-FAM-VFDAVTDVIIKNNLKECGLY is a fluorescein-labeled 20-residue peptide derived from the C-terminal region of the Gαi3 protein and is commonly referred to as HCAM-2 or F-Gαi3C20. Pertussis toxin catalyzes ADP-ribosylation of a cysteine near the C-terminus of inhibitory G-protein alpha subunits. This synthetic peptide preserves the relevant C-terminal cysteine-containing recognition region and has been used as a defined substrate in in vitro assays that measure the ADP-ribosyltransferase activity of pertussis toxin. |
Research Applications | - pertussis toxin ADP-ribosyltransferase assays
- acellular pertussis vaccine research
- Gαi/o peptide-substrate studies
- fluorescence/HPLC assay development
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Experimental Notes | The 6-FAM label supports fluorescence-based detection, but pertussis-toxin modification is an ADP-ribosylation reaction rather than proteolytic cleavage. Published assay formats commonly separate and quantify the ADP-ribosylated peptide product by HPLC. The peptide should therefore not be described as a conventional fluorogenic protease substrate. |
Selected References | - Development of an in vitro Biochemical Assay System for the Measurement of Residual Toxin Activities in Pertussis Toxin Containing Vaccines
- In Vivo Models and In Vitro Assays for the Assessment of Pertussis Toxin Activity
- A novel 96-well in vitro assay for assessing pertussis toxin activity in final products of acellular pertussis combination vaccines
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