Catalog Number | LT8934 |
Product Name | Glandular Kallikrein Fluorogenic Substrate, D-Val-Leu-Arg-AFC |
Product Quantity | 10 mg |
Sequence | vLR-AFC |
Molecular Weight | 597.7 |
Formula | C27H38F3N7O5 |
Purity | ≥95% |
Scientific Background | H-D-Val-Leu-Arg-AFC is a fluorogenic substrate used for glandular kallikrein and other arginine-directed serine-protease assays. Cleavage at the Arg-AFC bond releases AFC and increases fluorescence near 380/500 nm. The substrate provides a simple continuous readout for kallikrein activity, kinetic characterization and inhibitor studies. |
Research Applications | - glandular kallikrein activity assays
- kallikrein-kinin system research
- serine-protease kinetics
- kallikrein inhibitor screening
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Experimental Notes | Arg-AFC substrates may be processed by more than one trypsin-like protease. Enzyme specificity should therefore be established with purified enzymes, selective inhibitors or appropriate biological controls. |
Selected References | - Kallikreins as biomarkers and therapeutic targets in human disease
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