ERKtide ERK2 Substrate Peptide

ATGPLSPGPFGRR is a synthetic peptide substrate for extracellular signal-regulated kinase 2 (ERK2). The sequence contains a proline-directed serine phosphorylation motif characteristic of MAP kinase substrates and has been used in detailed kinetic studies of ERK2 catalysis. Its compact, sequence-defined format makes it useful for measuring ERK activity and evaluating catalytic mechanisms.

• ERK2 kinase activity assays
• MAP kinase phosphorylation studies
• ERK kinetic measurements
• MAPK inhibitor screening

This peptide is optimized as a biochemical substrate and should not be assumed to reproduce docking interactions present in full-length ERK targets. Quantitative assays should use consistent ATP, peptide and enzyme concentrations.

1. Catalytic Reaction Pathway for the Mitogen-Activated Protein Kinase ERK2
2. Two Rate-Limiting Steps in the Kinetic Mechanism of the Serine/Threonine Specific Protein Kinase ERK2