Elastase/Trypsin Fluorogenic Substrate, (Z-AR)2Rh1102HCl

(Z-Ala-Arg)2Rh110·2HCl is a rhodamine 110-based fluorogenic substrate for elastase, trypsin and related serine-protease activity measurements. Cleavage of the peptide groups releases fluorescent rhodamine 110, producing a strong signal suitable for continuous or endpoint assays. Rhodamine-based dipeptide substrates were developed as highly sensitive reporters for serine proteinases.

• elastase activity assays
• trypsin activity assays
• serine-protease inhibitor screening
• fluorogenic protease assay development

The released Rh110 signal is monitored near 497 nm excitation and 527 nm emission. This is not an isozyme-specific substrate, so purified-enzyme or inhibitor controls are recommended when assigning protease identity.

1. New class of sensitive and selective fluorogenic substrates for serine proteinases. Amino acid and dipeptide derivatives of rhodamine