Cys-TAT (47-57)

CYGRKKRRQRRR-NH2 is a cysteine-extended, C-terminally amidated form of the HIV-1 Tat(47-57) cell-penetrating peptide. The N-terminal cysteine provides a thiol handle for selective conjugation to proteins, oligonucleotides, fluorophores, lipids or other cargo while retaining the arginine-rich Tat transduction sequence.

• thiol-selective TAT conjugation
• cell-penetrating peptide delivery studies
• oligonucleotide and peptide conjugates
• carrier-peptide assay development

The N-terminal cysteine can oxidize to disulfides and should be handled under conditions appropriate for the intended conjugation chemistry. Conjugation can alter cell uptake relative to free TAT.

1. Cellular Uptake of Antisense Morpholino Oligomers Conjugated to Arginine-Rich Peptides
2. Single molecule sensitive multivalent polyethylene glycol probes for RNA imaging