Cys(Npys)-R9 Cell-Penetrating Peptide

Cys(Npys)-R9 Cell-Penetrating Peptide is an arginine-rich cell-penetrating peptide based on nona-arginine (R9), supplied with an N-terminal cysteine protected as an Npys derivative and a C-terminal amide.

Arginine-Rich Cell-Penetrating Peptides

Short oligomers of arginine are widely used as model CPPs. Their guanidinium-rich side chains interact strongly with anionic cell-surface molecules and membrane components, and they are commonly investigated for delivery of macromolecular or nanoparticle cargos.

Cys(Npys) Functional Group

The Npys-protected cysteine is a chemically activated thiol configuration that can be used in disulfide-exchange strategies. This allows an R9 CPP module to be coupled to a second thiol-containing peptide or cargo through a reducible disulfide linkage.

• CPP-cargo conjugation
• Reducible disulfide delivery constructs
• Cellular uptake studies
• Peptide translocation research

References

Futaki S, et al. Stearylated arginine-rich peptides: a new class of transfection systems. Bioconjug Chem. 2001.
doi: 10.1021/bc015508l.
Public source / publication

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Research Use Only. Not for human, diagnostic, clinical, or therapeutic use.

Cys(Npys)-R9 Cell-Penetrating Peptide is a 16-residue synthetic peptide with the sequence Cys-Asn-Pro-Tyr-Ser-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Asn-His. C-terminal amidation removes the terminal carboxylate charge. The sequence contains 1 cysteine residue, providing potential thiol/disulfide chemistry when the thiol is available; contains 0 Lys and 9 Arg residues, contributing cationic character. These sequence-derived properties describe the reagent chemically; no specific receptor, enzyme, pathway, disease association, or biological activity is assigned without product-specific experimental evidence.

• thiol-selective conjugation or immobilization studies
• LC-MS/HPLC analytical method development
• sequence-specific assay controls

Sequence-derived chemical properties support reagent selection and experimental planning but do not establish biological function. Solubility, aggregation, adsorption, conjugation efficiency, and assay performance should be validated under the intended experimental conditions.