Cynomolgus CD27 Ligand/CD70 Protein (LTP10235)

Product Name
Cynomolgus CD27 Ligand/CD70 Protein (LTP10235)
Catalog Number
LTP10235
Quantity
100ug
Price
$473 In stock
Background
CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7.  CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Description
Recombinant Cynomolgus CD27 Ligand/CD70 Protein is expressed from Expi293 with His tag at the N-terminal. It contains Gln39-Pro194.
Target
CD27 Ligand/CD70
Species
Cynomolgus
Gene/Protein ID
G7PYU6-1
Exact Sequence
Gln39-Pro194
Molecular Weight
The protein has a predicted MW of 18.4 kDa. Due to glycosylation, the protein migrates to 60-90 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Cynomolgus CD27 Ligand, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque CD27, hFc Tag with the EC50 of 70.8ng/ml determined by ELISA. See testing image for detail.
Tag
N-His
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE;
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Cynomolgus CD27 Ligand/CD70 Protein (LTP10235)
  • 5 Units in Stock
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