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The peptide sequence EQKLISEEDL corresponds to the c-Myc tag, a widely utilized 10-amino-acid epitope derived from the human c-Myc proto-oncogene. This tag is extensively employed in molecular biology for the detection, purification, and analysis of recombinant proteins.
Functional Overview
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Origin: The c-Myc tag is derived from amino acid residues 410–419 of the human c-Myc protein, a nuclear transcription factor involved in cell cycle regulation and apoptosis.
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Sequence: EQKLISEEDL
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Molecular Weight: Approximately 1.2 kDa.
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Applications:
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Protein Detection: The c-Myc tag is recognized by specific monoclonal antibodies, such as the 9E10 clone, facilitating detection through techniques like Western blotting, immunofluorescence, and immunoprecipitation.
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Protein Purification: c-Myc-tagged proteins can be purified using affinity chromatography methods that exploit the high-affinity interaction between the tag and anti-c-Myc antibodies.
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Protein Localization and Interaction Studies: The tag aids in studying protein localization within cells and interactions with other proteins.
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Versatility: Due to its small size, the c-Myc tag can be fused to either the N-terminus or C-terminus of target proteins without significantly altering their function or localization.
Research and Development
The c-Myc tag has been instrumental in various research applications:
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Epitope Tagging: It serves as a model epitope tag in studies aiming to understand protein expression and function.
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Antibody Production: Synthetic peptides corresponding to the c-Myc tag are used to generate monoclonal and polyclonal antibodies, which are critical tools in protein research.
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Biotinylation: Biotinylated versions of the c-Myc tag are available, allowing for enhanced detection and purification using streptavidin-based systems.
Considerations and Limitations
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Elution Conditions: While the c-Myc tag can be used for protein purification, elution often requires low pH conditions, which could negatively affect protein functionality.
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Secretory Pathway Interference: It is not recommended to fuse the c-Myc tag directly behind the signal peptide of a secretory protein, as it can interfere with translocation into the secretory pathway.
The EQKLISEEDL sequence, known as the c-Myc tag, is a valuable tool in molecular biology for the detection and purification of recombinant proteins. Its small size and the availability of high-affinity antibodies make it an ideal choice for various protein research applications.
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