Catalog Number | LT8788 |
Product Name | CDK7/9 CTD Peptide Substrate, YSPTSPSYSPTSPSYSPTSPSKKKK |
Product Quantity | 1 mg |
Sequence | YSPTSPSYSPTSPSYSPTSPSKKKK |
Molecular Weight | 2690.0 |
Formula | C120H185N29O41 |
Purity | ≥95% |
Scientific Background | YSPTSPSYSPTSPSYSPTSPSKKKK is a synthetic peptide substrate containing three tandem YSPTSPS repeats from the C-terminal domain (CTD) consensus motif of RNA polymerase II followed by four lysines. The repeated Ser-Pro/Thr-Ser-Pro motifs provide phosphorylation sites for transcription-associated cyclin-dependent kinases including CDK7 and CDK9. This defined peptide is useful for biochemical studies of CTD-directed kinase activity and transcriptional kinase inhibition. |
Research Applications | - CDK7 kinase activity assays
- CDK9 kinase activity assays
- RNA polymerase II CTD phosphorylation studies
- transcriptional kinase inhibitor screening
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Experimental Notes | The peptide contains multiple potential serine and threonine phosphorylation sites, so reaction products may be heterogeneous. Site-specific analysis may require phosphopeptide mapping or mass spectrometry. |
Selected References | - Inhibition of NF-kB-Mediated Signaling by the Cyclin-Dependent Kinase Inhibitor CR8 Overcomes Prosurvival Stimuli to Induce Apoptosis in Chronic Lymphocytic Leukemia Cells
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