Calpain/Proteasome Fluorogenic Substrate, Suc-LLVY-AMC

Suc-LLVY-AMC is a fluorogenic tetrapeptide substrate used for chymotrypsin-like protease activity measurements, including calpains and the 20S proteasome. Proteolytic cleavage releases AMC and generates a fluorescence signal that can be monitored continuously. Because multiple protease systems can hydrolyze LLVY-AMC substrates, the reagent is best used with selective inhibitors or purified enzymes when assigning activity to a specific protease.

• calpain activity assays
• 20S proteasome activity studies
• chymotrypsin-like protease assays
• protease inhibitor screening

AMC is typically monitored near 353 nm excitation and 442 nm emission. Protect the substrate from light and use pathway-selective inhibitors when measuring activity in complex lysates.

1. Polyubiquitin linkage profiles in three models of proteolytic stress suggest the etiology of Alzheimer disease
2. The JAMM motif of human deubiquitinase Poh1 is essential for cell viability