Kemptide primarily used in Kinase Assays (cyclic AMP-dependent protein kinase). Kemptide is a synthetic substrate for protein kinase A. This phosphate acceptor is a basic serine-containing heptapeptide corresponding to a sequence from pig liver pyruvate kinase. It is phosphorylated and activated after microinjection into Xenopus oocytes.
Kemptide is a synthetic heptapeptide with the sequence Leu-Arg-Arg-Ala-Ser-Leu-Gly (LRRASLG). It serves as a model substrate for cAMP-dependent protein kinase (PKA), facilitating the study of PKA activity and the development of kinase assays. The peptide is derived from a phosphorylation sequence of pig liver pyruvate kinase, a natural PKA substrate. Kemptide has a Km value of approximately 16 µM, indicating its affinity for PKA.
The biotinylated form of Kemptide, BIOTIN-LRRASLG, includes a biotin group at the N-terminus, enabling easy immobilization on streptavidin-coated surfaces and facilitating detection in various assays.
BIOTIN-LRRASLG is primarily used in kinase assays to study PKA activity. Its applications include:
Kinase Assays: As a synthetic substrate for PKA, it aids in evaluating kinase activity and screening for inhibitors.
Cell Signaling Studies: By monitoring the phosphorylation of Kemptide, researchers can investigate PKA-mediated signaling pathways.
Assay Development: The biotinylated form allows for the development of assays that require immobilization and detection of the substrate.
| 