Autocamtide-2 CaMKII Substrate

Autocamtide-2 (AC-2), KKALRRQETVDAL, is a well-established peptide substrate for Ca2+/calmodulin-dependent protein kinase II (CaMKII). It is derived from the CaMKII autophosphorylation region and contains a threonine phosphoacceptor in a recognition sequence efficiently phosphorylated by activated CaMKII. AC-2 is frequently used as a positive control and quantitative substrate in CaMKII activity assays.

• CaMKII activity assays
• Ca2+/calmodulin signaling research
• CaMKII positive-control phosphorylation
• kinase kinetics and inhibitor studies

CaMKII activity can be measured under Ca2+/calmodulin-dependent or autonomous conditions. Experimental interpretation should distinguish these activation states and control for other kinases when crude lysates are used.

1. Activation State-Dependent Substrate Gating in Ca2+/Calmodulin-Dependent Protein Kinase II
2. CaMKII Inhibition Attenuates Distinct Gain-of-Function Effects Produced by Mutant Nav1.6 Channels and Reduces Neuronal Excitability