|
The peptide sequence SRETSEKFKLLFQSYNVND corresponds to the ARA70 coactivator peptide, which is utilized in Time-Resolved Fluorescence Resonance Energy Transfer assays to study nuclear receptor interactions. Specifically, this peptide is employed to investigate the binding dynamics between nuclear receptors and coactivator proteins, providing insights into gene transcription mechanisms.
Role in Nuclear Receptor Coactivator Assays
In the context of TR-FRET assays, the ARA70 coactivator peptide is conjugated with a fluorescein moiety, enabling the detection of interactions between nuclear receptors and coactivators through energy transfer measurements. This setup allows researchers to quantify the binding affinity and kinetics of these interactions, which are pivotal in regulating gene expression. The peptide's sequence is designed to mimic the LXXLL motif, a characteristic feature of coactivator proteins that facilitates their association with nuclear receptors.
Applications in Research:
The fluorescein-labeled ARA70 peptide is instrumental in high-throughput screening assays aimed at identifying modulators of nuclear receptor activity. By assessing how various compounds influence the peptide's binding to nuclear receptors, researchers can discover potential therapeutic agents that modulate gene transcription pathways. Such studies are particularly relevant in the development of treatments for diseases where nuclear receptor signaling is dysregulated, including certain cancers and metabolic disorders.
The SRETSEKFKLLFQSYNVND peptide serves as a valuable tool in molecular biology for elucidating the mechanisms of nuclear receptor-coactivator interactions. Its application in TR-FRET assays facilitates the exploration of gene regulation processes and the discovery of novel pharmacological modulators targeting nuclear receptor pathways. | 