Technical Protocols
Overview of HLA-DRB1 and Its Role in the Immune System
Last Updated: March 2, 2026HLA-DRB1 Gene and Protein Function HLA-DRB1 is part of the HLA class II beta chain paralogs. The class II molecule is a heterodimer composed of an alpha (DRA) and a beta chain (DRB), both of which are anchored in the...
Improved identification of neoantigen-reactive T cells targeting both unique and common oncogenes for personalized cancer immunotherapy
Last Updated: March 2, 2026Adoptive cell transfer (ACT) of tumor-infiltrating lymphocytes (TILs) that target neoantigens can cause tumor regression in certain patients with metastatic epithelial cancer. However, identifying and utilizing neoantigen-reactive T cells for treatment remains challenging, and it is unclear if current detection...
Screening of Neoantigen HLA Complexes
Last Updated: February 27, 2026Mutated peptides, known as neoantigens, derived from a patient’s cancer genome can be targeted by T-cell immunity. However, identifying which peptides can be presented by MHC molecules and stimulate T cells has proven challenging. Existing algorithms can predict MHC binding...
Poly(2-oxazoline)s: The Emerging Frontiers of Biomedical Polymer Engineering
Last Updated: March 2, 2026The field of biomedical applications has been significantly enriched by the inclusion of polymers, which have opened new avenues in drug delivery, tissue engineering, implant fabrication, and biosensing. Polymers fused with pharmaceuticals can innovatively address numerous unmet medical needs, such...
linker-spacer-examples
Last Updated: February 27, 2026Linker/Spacer In the field of peptide synthesis, linkers play a crucial role by bridging the gap between various molecular entities, thus enabling the creation of complex peptides and proteins with desired functionalities. These linkers are not merely inert spacers; they...
A sample protocol using CPP for plasmid transfection
Last Updated: February 27, 2026Creating a cell-penetrating peptide (CPP) for transfection with DNA plasmids is a complex and specialized procedure that requires careful design and optimization. Below is a general protocol to give you an overview of the steps involved. Keep in mind that...
Does LifeTein offer antigen design assistance?
Last Updated: February 27, 2026Peptide Antigen Design for Antibody Production Designing peptide antigens for antibody production is a sophisticated process that leverages computational tools and bioinformatics to identify sequences within proteins that are likely to elicit a robust immune response. This process is crucial...
LifeTein Dot Blot protocol
Last Updated: February 27, 2026LifeTein Dot Blot protocol Dot Blot protocol Dot blot is similar to the Western blot technique. However, the proteins are spotted directly onto the membrane or paper for detecting and analyzing. This is a good technique to estimate protein concentration....