Antibody Services
Advancements in Labeled Peptide Synthesis: Strategies for Incorporating Biotin, FITC, and Other Tags
Last Updated: February 27, 2026The synthesis of labeled peptides, such as those tagged with biotin, FITC, or nanoparticles, plays a crucial role in a wide range of research applications, including binding studies and receptor cross-linking investigations. A novel strategy for producing these peptides involves...
Why is conjugation of the peptide to a carrier protein necessary?
Last Updated: February 27, 2026The process of linking a peptide to a carrier protein is a foundational technique in vaccine development and antibody production, enhancing the overall efficacy and response of the immune system to the antigen. This conjugation serves multiple critical functions in...
To save cost, can I split the purified peptide so that half is labeled with biotin and the remaining left unlabeled
Last Updated: February 27, 2026Can you split the purified peptide so that half the sample could be labeled with a biotin tag and the remaining is left unlabled. Do I need to quote for two peptides? This is for N terminal labeling. The label...
How to perform competitive ELISA?
Last Updated: February 27, 2026Performing Competitive ELISA with Peptides: A Step-by-Step Guide I. Introduction A. Brief Explanation of Competitive ELISA Competitive Enzyme-Linked Immunosorbent Assay (ELISA) is a powerful technique used in immunology and molecular biology to determine the concentration of an antigen in a...
How to Perform Immunohistochemistry Using Biotinylated Peptide?
Last Updated: February 27, 2026A peptide RK-10 is used to detect Programmed Death Ligand 1 (PD-L1) expressing tumors with immunohistochemistry. In order to detect PD-L1 in tissues, a biotin-conjugated version of peptide RK-10-Cy5 was used. Tissues were incubated with 15 µM biotinylated peptide for...
ELISA Protocol
Last Updated: February 27, 2026ELISA Protocol Coating the appropriate antigen to microplate 1. Dilute the antigen to a final concentration of 20 μg/ml in PBS. Fill the microwells of a Nunc Maxi-Sorp Immuno Plate with 50 μL of the diluted antigen. Note: Test samples...
ELISA standard curve
Last Updated: February 27, 2026ELISA standard curve Standards and the standard curve 1. Make up a stock solution of 0.08 ug of protein/100 ul of PBS and store at 4 C To make the stock: Use protein extracted from fresh hyphae that are nearly...
Western Blot: Technique, Theory, and Trouble Shooting
Last Updated: February 27, 2026Western Blot: Technique, Theory, and Trouble Shooting Western blotting is an important technique used in cell and molecular biology. Researchers can identify specific proteins from a complex mixture of proteins extracted from cells using Western Blot. A mixture of proteins...
Guidelines for the storage of different types of antibody
Last Updated: February 27, 2026Guidelines for the storage of different types of antibody Storage temperatures and conditions For many of our antibodies, freezing at -20 C or -80 C in small aliquots is the optimal storage condition. Aliquotting minimizes damage due to freezing and...