LifeTein Biotin-Ahx-LPETGS-NH2

Biotin-Ahx-LPETGS-NH₂ - Site-specific sortase substrate (biotinylated)

Biotin-Ahx-LPETGS-NH₂ is a small, soluble peptide substrate designed for efficient Sortase A–mediated transpeptidation. The canonical LPETG(S) recognition motif is presented with an aminohexanoic (Ahx) spacer and an N-terminal biotin, producing a compact reagent ideal for site-specific biotinylation, affinity capture, proximity labeling, and surface immobilization workflows.

Order this product now!

Key applications

• Proximity / cell–cell labeling: Used as the donor substrate in sortase-based proximity labeling (for example, LIPSTIC-style methods) to tag transient cell contacts for capture and downstream analysis.
• Site-specific protein and antibody labeling: Enables near-quantitative installation of a single biotin handle at defined positions on recombinant proteins or antibodies via Sortase A transpeptidation.
• Surface functionalization & biomaterials: Provides a covalent anchoring point for immobilizing proteins or regenerable bioactive coatings on sensors and implantable materials.
• Affinity capture & purification workflows: Acts as a capture or release handle in tag-free purification strategies that leverage biotin-streptavidin systems after enzymatic ligation.

Why choose Biotin-Ahx-LPETGS-NH₂

The Ahx spacer reduces steric hindrance between the biotin and the LPETG motif, improving enzymatic access and downstream streptavidin binding. This compact design is compatible with in-vitro enzymology, cell-surface labeling, and surface chemistry,making it a versatile reagent for research, diagnostics development, and materials science applications.

Representative references

1) LIPSTIC — cell-cell / immune-interaction labeling

Pasqual et al., (LIPSTIC method / monitoring T cell–dendritic cell interactions) — Describes LIPSTIC (Labeling Immune Partnerships by SorTagging Intercellular Contacts), a proximity-labeling method that uses sortase A + an LPETG peptide (often biotin- or fluorophore-conjugated) to record transient cell–cell contacts in vivo and recover interacting cells for downstream analysis. Biotin-Ahx-LPETGS is used as the donor substrate that SrtA transfers onto an acceptor cell bearing N-terminal glycine(s), enabling detection by streptavidin/flow or single-cell analysis. (https://pubmed.ncbi.nlm.nih.gov/29342141/)

Applications: mapping transient immune synapses, cell–cell interactome atlases, and coupling with single-cell multi-omics.

2) Site-specific protein/antibody labeling & bioconjugation

Cong et al., 2021 (Direct N- or C-terminal protein labeling via sortase) — Demonstrates near-quantitative sortase-mediated biotinylation/labeling of antibodies and proteins using glycine nucleophiles and biotinylated LPETG substrates. Biotin-LPETG (or Gly₃-biotin variants) are standard reagents to install streptavidin-compatible handles site-specifically. (https://pmc.ncbi.nlm.nih.gov/articles/PMC9595177/)

Applications: site-specific ADCs, diagnostic reagent production, and modular assembly of multivalent therapeutics (via streptavidin or click handles).

3) Sortase reviews & methodological landscape

Wu 2012; Morgan 2022 (reviews / challenges & engineering of sortases) — thorough reviews of sortase-mediated ligation, its enzyme mechanism (LPXTG cleavage between T–G), strengths, limitations (kinetics, reversibility, calcium dependence), and engineered variants/orthogonal sortases for multiplexed labeling. These reviews place biotin-LPETG peptides as common, validated substrates for biochemical assays and protein engineering. (https://pmc.ncbi.nlm.nih.gov/articles/PMC3314344/)

Applications: orthogonal multi-site labeling, improved in-cell ligations, and engineered sortases with higher activity/specificity.

4) Biomaterials & in-situ regeneration of bioactive coatings

Ham et al., Nat Commun 2016 (in-situ regeneration of bioactive coatings using sortase) — shows use of sortase to covalently attach peptides/proteins (via LPXTG motif) to surfaces and regenerate coatings. Biotinylated LPETG peptides are convenient anchors/handles for surface capture and for coupling to streptavidin-based assemblies.(https://www.nature.com/articles/ncomms11140)

Applications: dynamic/renewable coatings, sensor surfaces, and on-demand biofunctionalization in implantable devices.

5) Practical methods: biotinylated sortase approaches / purification tricks

Matsunaga et al., 2010 (BISOP — biotinylated-sortase self-cleavage purification) — an early method using biotinylated sortase substrates to improve tag-free protein purification and recovery; demonstrates practical bench workflows that make biotin-LPETG reagents useful beyond detection (e.g., purification or capture). (https://pmc.ncbi.nlm.nih.gov/articles/PMC2901362/)

Applications: robust manufacturing workflows for site-specific capture/purification of therapeutic proteins.

For technical support, suggested reaction conditions, or bulk ordering information,please contact our scientific support team — we can provide recommended sortase ratios,buffer conditions, and handling tips tailored to your workflow.