Product Name | Z-LLE-AMC |
Product Quantity | 5 mg |
Catalog Number | LTC-003 |
Molecular Weight | 664.8 |
Formula | C35H44N4O9 |
Sequence | Z-Leu-Leu-Glu-AMC, Z-Leu-Leu-Glu-7-amido-4-Methylcoumarin |
Purity | >95% |
CAS Number | 348086-66-8 |
Product Description | Proteasome Substrate II, Z-LLE-AMC, is a fluorogenic substrate for the peptidylglutamyl-peptide hydrolyzing (caspase-like) activity of the proteasome. Z-LLE-AMC's Caspase-like activity can be quantified by fluorescent detection of free AMC (7-amino-4-methylcoumarin). AMC is excited at 340-360 nm and emits at 440-460 nm. Z-LLE-AMC can be stimulated in the presence of Mg2+ ions and rapidly inactivated by N-acetylimidazoleis. |
Scientific Background | Z-LLE-AMC is a 6-residue synthetic peptide with the sequence Leu-Leu-Glu-Leu-Leu-Glu. The sequence has a relatively high hydrophobic-residue fraction that can influence aqueous solubility and surface adsorption. These sequence-derived properties describe the reagent chemically; no specific receptor, enzyme, pathway, disease association, or biological activity is assigned without product-specific experimental evidence. |
Research Applications | - LC-MS/HPLC analytical method development
- sequence-specific assay controls
|
Experimental Notes | Sequence-derived chemical properties support reagent selection and experimental planning but do not establish biological function. Solubility, aggregation, adsorption, conjugation efficiency, and assay performance should be validated under the intended experimental conditions. |
