Woodtide DYRK Kinase Substrate

Woodtide, KKISGRLSPIMTEQ-NH2, is a Forkhead-derived peptide substrate used to measure dual-specificity tyrosine-phosphorylation-regulated kinase (DYRK) activity. The core sequence corresponds to the region surrounding a DYRK phosphorylation site in Forkhead/FKHR, while two N-terminal lysines facilitate phosphocellulose capture in classical radiometric kinase assays. Woodtide is widely used for DYRK1A, DYRK1B and related kinase studies.

• DYRK1A/DYRK1B kinase assays
• DYRK substrate specificity studies
• kinase inhibitor screening
• radiometric phosphocellulose assays

Woodtide is a biochemical assay substrate rather than a full-length Forkhead protein. Its added N-terminal lysines are part of the assay design and should be retained when comparing published kinetic or inhibition data.

1. Differing substrate specificities of members of the DYRK family of arginine-directed protein kinases
2. Activation-loop autophosphorylation is mediated by a novel transitional intermediate form of DYRKs