Tyrosine Kinase Peptide 1

KVEKIGEGTYGVVYK is a sequence-defined protein tyrosine kinase substrate derived from a CDC2-related region and has been used as a generic substrate in protein tyrosine kinase assays. The peptide contains tyrosine residues that permit phosphorylation-dependent enzyme activity measurements and comparison of kinase or inhibitor conditions.

• protein tyrosine kinase activity assays
• Src-family kinase substrate studies
• kinase inhibitor screening
• phosphorylation assay development

This is the unconjugated free-acid peptide. It is chemically distinct from the 5-TMR-labeled form and should be retained as the unlabeled reference substrate when fluorescence labeling could affect assay behavior.

1. Identification and characterization of a synthetic peptide substrate for Src-family protein tyrosine kinases